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At this point, you must return to Prague and speak with Ecaterina in order to continue in your journey to rescue Anezka. Make your way to the University, where Ecaterina will tell you of the recent happenings in Prague. It seems that Vysehrad Castle is the scene of an uprising against the vampires and is most likely where you'll find Anezka.

I highly recommend visiting your haven to allocate any experience before besieging Vysehrad Castle because of the fact that you will no longer have Serena or Wilhelm at your disposal after you've finished with the castle. Once you've allocated your experience, stock up on blood and head towards Vysehrad Mountain to confront Anezka's captors.